Figure 2

Major Functional Response Trends across the Eye

(A) Kernel amplitudes of all dendritic (top) and somatic (bottom; y-flipped) ROIs, shown for the maximal amplitude kernel of each ROI irrespective of color. For a breakdown by color, see Figures S2G and S2H. The arrowhead emphasizes a relative reduction in OFF responses at the level of somata. Chi-square with Yates correction for On:Off distributions dendrites versus somata: p < 0.00001.

(B) Prominence of different color and polarity responses among dendrites (top row) and somata (bottom row), plotted across visual space. In each case, all kernels that exceeded a minimum amplitude of 10 SDs were included. Scale bars in percent of dendritic/somatic ROIs that were recorded in a given section of the eye such that the percentages of On, Off, and non-responding (<10 SD) add to 100% are shown.

(C–E) Schematic illustrating how dendritic ROIs from different parts of the eye and IPL depth (C) were mapped into a 2D “Eye-IPL” map (D), which can then also be analyzed over time (E). Note that this involved “cutting” the circular range of eye positions such that the ventral retina is represented at either edge along the 2-projections’ x axis.

(F and G) Example snapshots of mean responses to chirp stimulation (cf. Figure 1G) mapped into an eye-IPL map as schematized above (C–E). Data can be plotted as time traces for a given region of the eye and IPL (F; r_1,2 as indicated in G) or alternatively as a time-frozen snapshot of activity across the eye and IPL at different points in time (G; t_1–4 as indicated in F). See also Videos S2 and S3.

(H–J) As (F) and (G) but instead showing mean kernels across the four spectral wavebands, where (H) and (I) are mean and max-scaled mean kernels for Eye-IPL regions r_1,2 (as in F), respectively. (J) shows each kernel’s full Eye-IPL map at two time points t_5,6 as indicated in (H) and (I) (see also Figure S2I). In the color scale bar, 0 equates to the baseline of each bin’s kernel and 1/−1 to their respective maximum or minimum (cf. I). See also Video S4.

(K and L) Distribution of central frequencies (STAR Methods) of dendritic (top) and somatic (bottom; inverted y axis) kernels in the four wavebands, separated into On (K) and Off (L) kernels. Wilcoxon rank-sum test, 1 tailed with correction for multiple comparisons for all pairwise comparisons between same polarity distributions of spectral centroids, is shown. Dendrites: all p < 0.001 except R_Off versus G_Off (p = 0.0011) and G_On versus B_On (p = 0.69). Somata: all p < 0.001 except R_On versus U_On (p = 0.00101), R_Off versus G_Off (p = 0.033), G_On versus B_On (p = 0.045), B_On versus U_On (p = 0.064), R_On versus B_On (p = 0.25), and R_On versus G_On (p = 0.57).