FIGURE

Fig. 5

ID

ZDB-FIG-200728-46

Publication

Brandt et al., 2020 - Core Hippo pathway components act as a brake on Yap/Taz in the development and maintenance of the biliary network

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Fig. 5

Hepatocyte Hippo signaling is crucial for larval survival and functions non-cell-autonomously to affect the intrahepatic and extrahepatic biliary system. (A,B,C-C?,F-F?) Whole-mount GFP staining in wild-type and sav1?/? larvae. (A?,B?,D-D?,G-G?) Annexin A4 staining of gallbladder and intrahepatic biliary cells. Gb, gallbladder; EHD, extrahepatic duct; EPD, extrapancreatic duct; IBD, intrahepatic bile duct. (A?,B?,E-E?,H-H?) Merged images with green representing GFP and magenta representing annexin A4. (A-B?) Larvae at 80?hpf (n=3 wild type, lfabp:eGFP-2a:Sav1+ and sav1?/?, lfabp:eGFP-2a:Sav1+). (C-E?) Larvae at 8?dpf (n=8 wild type, lfabp:eGFP-2a:Sav1+; n=7 sav1?/?, lfabp:eGFP-2a:Sav1+; n=3 wild type, non-transgenic sibling; n=5 sav1?/?, non-transgenic sibling). (F-H?) Fish at 16?dpf (n=12 wild type, lfabp:eGFP-2a:Sav1+; n=12 sav1?/?, lfabp:eGFP-2a:Sav1+; n=8 wild type, non-transgenic sibling; n=4 sav1?/?, non-transgenic sibling). Scale bars: 100 ?m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sav1^mw95/mw95 sav1^mw95/mw95; mw97Tg
Observed In:	gall bladder intrahepatic bile duct
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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