ZIP4 silencing enhances radiosensitization in vitro.a Cell growth in low and high concentration of zinc. b Inhibition of cell proliferation following exposure to various doses of Co60 was evaluated by the MTT assay (P < 0.01). c Images and quantification of the number of colonies formed from cells treated with 0, 2, 4, 6, and 8 Gy (P < 0.05). d Flow cytometry analysis of apoptosis using annexin V and propidium iodide in cells treated with various doses of radiation (0, 2, 4, 6, and 8 Gy). e Flow cytometric analysis of the cell cycle in cells treated with various doses of radiation. f Protein expression levels of cleaved caspase-3, cleaved caspase-9, Bax, and Bcl-2 in cells treated with a 6 Gy dose of radiation. Experiments were repeated three times. The data are represented as means ± SD from three independent experiments. *P < 0.05
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