|
ZIP4 silencing prevents C666-1 tumor cell invasion, dissemination, and metastasis in a zebrafish model and a lung metastasis mouse model.a, b Red fluorescence-labeled tumor cells were injected into the perivitelline space of 48 h postfertilization embryos and tumor cell invasion, dissemination, and metastasis were detected using confocal microscopy at day 3 post injection. Yellow arrows indicate primary tumors. White arrows indicate disseminated tumor foci (Scale bar = 100 μm). c Quantification of disseminated tumor foci (n = 40 per group), P < 0.01. d Representative images of the mouse lungs. e The average number of tumor metastatic nodules in the lungs of mice xenografted with C666-1, LVRH C666-1 cells, and Sh-ZIP4 C666-1 cells is provided (N = 7; P < 0.01). f Representative views of lung tissue sections from each group are shown (Scale bar = 50 μm). The experiments were performed in triplicate. The data are represented as means ± SD from three independent experiments
|
