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vip, npb, and trh expression domains in mamillary hypothalamic regions are lost in bsx mutant embryos. Lateral (a,b,e,f,i,j) and dorsal views (c,d,g,h,k,l) of the ventral forebrain in 3 dpf embryos stained by in situ hybridization using probes as indicated. All images are minimum intensity projections of 40 brightfield focal planes (distance 1 μm). Scale bar in (a) for all images: 50 μm. Anterior at left. Schematics to the right show location of vip (blue), npb (magenta), or trh (red) expressing cells as observed by stainings and indicate the focal planes which are shown in dorsal view pictures and schematics. Cells with Bsx-dependent expression are circled. Numbers n (phenotype shown)/n (total analyzed) as indicated.
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