ZFIN ID: ZDB-FIG-200619-50
Sun et al., 2020 - ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling. Nature communications   11:2984 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: 5-9 somites to Long-pec

Fig. 8 Loss of <italic>adnp</italic> leads to the defective neural development in zebrafish embryos.

a CRISPR/Cas9 gRNA design and the genotyping result of the adnpa mutant allele. b gRNA design and the genotyping result of adnpb mutant allele; c representative morphology of 2 dpf control and adnpa adnpa double mutants. Black arrow showing the obvious cell death (black area) in head region of mutant embryos. d 3D Z-stack image showing the reduced expression of HuC/D in brain of 36 hpf control and double mutant zebrafish embryos. Arrows show the distance between the convex tip of the eye cups. e GFP fluorescence signal showing the reduced elavl3-GFP levels in adnpa adnpb deficient embryos. f Whole-mount in situ hybridization (WISH) images for the indicated neural markers for 2 dpf control and double mutant embryos. g WISH images for the indicated neural markers for 13 hpf control and double mutant embryos. h WISH images for the indicated neural markers for 24 hpf control and double mutant embryos. Black arrows pointing to where expression levels were reduced. All experiments were repeated at least two times, and similar results were obtained. Scale bars: 200 μm in (c), 100 μm in (dh).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx5a WT standard conditions Long-pec brain ISH
adnpaihb804/ihb804; adnpbihb805/ihb805 standard conditions Long-pec brain ISH
EGFP adnpaihb804/ihb804; adnpbihb805/ihb805; knu3Tg standard conditions Prim-25 brain IFL
knu3Tg standard conditions Prim-25 brain IFL
neurod1 WT standard conditions Long-pec brain ISH
adnpaihb804/ihb804; adnpbihb805/ihb805 standard conditions Long-pec brain ISH
phox2a WT standard conditions Long-pec brain ISH
adnpaihb804/ihb804; adnpbihb805/ihb805 standard conditions Long-pec brain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
adnpaihb804/ihb804; adnpbihb805/ihb805 standard conditions 5-9 somites central nervous system development process quality, normal
Prim-5 central nervous system development decreased process quality, abnormal
Long-pec brain dlx5a expression decreased amount, abnormal
Long-pec brain neurod1 expression decreased amount, abnormal
Long-pec brain phox2a expression decreased amount, abnormal
Long-pec brain cell death increased process quality, abnormal
Long-pec post-vent region curved, abnormal
Long-pec whole organism morphology, normal
adnpaihb804/ihb804; adnpbihb805/ihb805; knu3Tg standard conditions Prim-25 brain EGFP expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.