FIGURE

FIGURE 4

ID
ZDB-FIG-200619-18
Publication
Varga et al., 2020 - Tissue-Specific Requirement for the GINS Complex During Zebrafish Development
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FIGURE 4

Gins1 deficient and Gins1, Gins2 double-deficient embryos show comparable cell death phenotypes in eyes and tecta. (A,B) The gins1elu11 frameshift allele created through genome editing shows retinal and tectal apoptosis at 2 dpf, similar to other CMG mutants. (C) Sanger sequencing of gins1elu11/elu11 embryos shows the presence of the homozygous c.250_251delCT mutation. (D,E) Phenotype of gins1 mutant embryos and their siblings injected with 100 μM gins2MO. (F) Western blot analysis of protein lysates from 2 dpf control and gins1elu11/elu11 embryos probed with Gins1 and γ-tubulin antibodies. (G) Western blot analysis of protein lysates from wildtype embryos at the indicated stages probed with Gins1 and γ-tubulin antibodies. Scale bar: 250 μm.
Expression Data
Gene:
Fish:
Anatomical Term:
Stage Range: 8-cell to Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol
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