Fig. S4

Explore the radioprotective effects of metformin on lower dose radiation (1 and 3 Gy). Zebrafish embryos at 3 hpf were exposed to MET (2 mg/ml) before radiation challenge (1 and 3 Gy). Then the hatching process and deformation of zebrafish larvae were observed (Fig. A-C, 1 Gy radiation; Fig. D-I, 3 Gy radiation). (A) Effects of MET (2 mg/ml) on the rate of larvae with normal notochord radiation exposure. (B) The proportion of each level of spine bending in the total curvature between IR and MET group. (C) The proportion of each level of pericardial edema in the total pericardial edema between IR and MET group. (D) Survival rate at 120 hpf. (E) Curve of hatching speed, n=3 per group; *P < 0.05, **P < 0.01, ***P < 0.001 represent Ctrl group compared with TAI group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 represent MET group compared with TAI group; Student’s t-test. (F) Effects of MET (2 mg/ml) on the rate of larvae with normal notochord radiation exposure. (G) Effects of MET (2 mg/ml) on the rate of larvae with normal heart after radiation exposure. (H) The proportion of each level of spine bending in the total curvature between IR and MET group. (I) The proportion of each level of pericardial edema in the total pericardial edema between IR and MET group. Each experiment was repeated at least three times and each treatment group contains 102 zebrafish larvae divided into nine parallel groups. The data were presented as mean ± SEM. Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001.