FIGURE

Figure 3.

ID

ZDB-FIG-200612-4

Publication

Tunbak et al., 2020 - Whole-brain mapping of socially isolated zebrafish reveals that lonely fish are not loners

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Figure 3.

Changes in baseline brain activity following isolation.

(A) Images of two areas that show strong c-fos activation in fully isolated fish independent of social stimuli (optic tectum and posterior tuberal nucleus (PTN)). Schematics of the horizontal sections and corresponding DAPI image are shown in the left panels. One plane of the 3D mask regions used for subsequent analysis is indicated (green). Images of Fully isolated fish c-fos neuronal activity, calculated as average differences between fully isolated (Fi) fish and normally raised fish without social cues (nsc) are shown in the right panels. Scale bar 200 μm. (B) Summary graphs showing the normalised c-fos expression in the optic tectum and PTN 3D masks for each experimental condition: non social cue (nsc), pro-social (+S) and anti-social (-S) for all the controls (C), fully isolated (Fi), and partially isolated (Pi) fish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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