Fig. 5 S2
Optogenetic stimulation of the KalTA4u508 pretectal population induces hunting with short latency.(A) Digital micromirror device (DMD) setup used for patterned illumination of pretectum and behavioural tracking in partially tethered larvae. (B) Opsin expression in a 6 dpf KalTA4u508;UAS:CoChR-tdTomato larva (image acquired with 2-photon microscope). Coloured boxes indicate AF7-pretectal regions targeted for stimulation. A, anterior; L, left; P, posterior; R, right. Scale bar, 50 µm. (C) Frames from an optogenetically induced hunting event in a tethered 7 dpf KalTA4u508;UAS:CoChR-tdTomato larva. Times relative to light onset. Eye position is marked by blue lines. (D) Hunting response probability of opsin-expressing (blue) and control, opsin-negative (grey), larvae (mean ± SEM, N = 7 fish per group). Between 6 and 9 trials per stimulated region were performed in each animal. (E) Mean latency of hunting responses for left (blue) and right (red) pretectal stimulations. (F) Percentage of hunting responses in which the first tail movement was contralaterally directed. |