FIGURE

Figure 1

ID

ZDB-FIG-200423-48

Publication

Gong et al., 2020 - The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish

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Figure 1

Sex-determining region Y box 2 (Sox2) expression analyses in the spinal cord and motor neurons. (A) At 20 hpf, the in situ hybridization signal of Sox2 is localized in the spinal cord. (A′) The magnified figure of the region squared in dashed line. (A″) The trunk transverse section of the embryos. (B) Sox2 is expressed in the spinal cord and the deposited neuromasts. (B′) The magnified figure of the region squared in dashed line. Scale bar = 200 μm. (C) The procedure of the motor neurons and endothelial cells sorting and RT-PCR. (D) The results of the RT-PCR on Mnx1-GFP sorted cells. Sox2 is expressed in the selected neuron cells from the Tg(Mnx1:EGFP) line. (E) The result of the RT-PCR on Kdrl-EGFP sorted cells. No signals of Sox2 and mnx1 are detected in the GFP-positive cells sorted from the Tg(kdrl:EGFP) line.

Expression Data

Genes:	mnx1 sox2
Fish:	WT ml2Tg s843Tg
Anatomical Terms:	brain motor neuron neuromast posterior lateral line primordium spinal cord spinal cord neural tube
Stage Range:	20-25 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Mol. Neurosci.