FIGURE

Fig. 7

ID

ZDB-FIG-200421-10

Publication

Liu et al., 2020 - Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development

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Fig. 7

Reduced dlx2a expression in pharyngeal arches 3–4 in pax1a; pax1b morphants beginning at 22 hpf. Similar dlx2a expression levels were detected in three migrating streams (S1, S2, S3) of cranial neural crest cells in uninjected (C), 0.75 ng pax1a MO-injected pax1b−/− mutant embryos (D), uninjected wild types (WT; A) or 0.75 ng pax1a MO-injected wild types (B) during 18–22 s stages. Similar dlx2a expression levels in three migrating streams of cranial neural crest cells were observed in wild types and pax1a; pax1b morphants at 18 hpf (E, L) and 20 hpf (F, M). However, reduced dlx2a expression (asterisk) was identified in pharyngeal arches (pa) 3–4 of pax1a; pax1b morphants compared to wild-type embryos at 22 hpf (G, N), 24 hpf (H, O), 26 hpf (I, P), 28 hpf (J, Q) and 30 hpf (K, R). Scale bars represent 100 μm.

Expression Data

Gene:	dlx2a
Fish:	WT pax1b^as37/as37 WT + MO1-pax1a pax1b^as37/as37 + MO1-pax1a WT + MO1-pax1a + MO3-pax1b
Knockdown Reagents:	MO1-pax1a MO3-pax1b
Anatomical Terms:	migratory cranial neural crest cell pharyngeal arch 3 pharyngeal arch 4 pharyngeal arch 5
Stage Range:	14-19 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pax1a + MO3-pax1b
Knockdown Reagents:	MO1-pax1a MO3-pax1b
Observed In:	pharyngeal arch 3 pharyngeal arch 4 pharyngeal arch 5
Stage Range:	26+ somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 161, Liu, Y.H., Lin, T.C., Hwang, S.L., Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development, 103598, Copyright (2020) with permission from Elsevier. Full text @ Mech. Dev.