Fig. 2

Maturation of choriocapillaris leads to increased perfusion and reduced leakage. A: Confocal micrographs of the posterior eye of Kdrl:EGFP; Gata1:DsRed double transgenic embryos (endothelium shown in green and erythroblasts shown in red) at 48?120 hpf. Boxed regions are shown in magnified images below. White arrowheads points to erythrocytes. Size bar indicates 50??m hpf, hours post fertilization. D: Quantification of the number of erythrocytes in the choriocapillaris between 48 and 120 hpf from the experiment shown in A. n = 10?15 embryos, ***:p < 0.001. C: Confocal micrographs of Kdrl:EGFP embryos (endothelium shown in green) at different time points between 48 and 120 hpf, 1?h after injection with rhodamine-conjugated 70?kDa dextran via the common cardinal vein (dextran (Dex) shown in red). Boxed regions are shown in magnified images below. Yellow arrowheads points to dextran leaked into the avascular area while white arrowheads points to dextran contained within the vascular lumen. Size bar indicates 50??m. D: Quantification of perfusion in the choriocapillaris between 48 and 120 hpf. n = 10?15 embryos, ***:p < 0.001. E: Quantification of the dextran leakage between 48 and 120hpf. n = 10?15 embryos, ***:p < 0.001.