FIGURE

Fig. 4

ID

ZDB-FIG-200413-52

Publication

Chestnut et al., 2019 - Zebrafish etv2 knock-in line labels vascular endothelial and blood progenitor cells

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Fig. 4

A comparison of etv2 ci32Gt+/?; UAS:GFP, TgBAC(etv2:GFP), Tg(?2.3etv2:GFP) fluorescence pattern (in kdrl:mCherry background, top row) and etv2 mRNA expression analyzed by in situ hybridization (ISH) at 24 hpf. etv2 ci32Gt+/?; UAS:GFP expression is apparent throughout the entire vasculature, red blood cells (RBC), and macrophages (MF). TgBAC(etv2:GFP) shows similar expression pattern, and also shows nonspecific expression in the neural tube. A?C, Merged mCherry and GFP channels; D?F, GFP channel; G,H, magnified images of the trunk region in, A and B. I, ISH analysis for etv2 mRNA expression at 24 hpf. DA, dorsal aorta; PCV, posterior cardinal vein; ISV, intersegmental vessels

Expression Data

Genes:	EGFP etsrp mCherry
Fish:	WT ci1Tg; ci5Tg ci5Tg; zf372Tg etsrp^ci32Gt/+; ci5Tg/+; nkuasgfp1aTg
Anatomical Terms:	blood vessel endothelial cell cranial vasculature dorsal aorta intersegmental vessel macrophage notochord nucleate erythrocyte posterior cardinal vein
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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