ZFIN ID: ZDB-FIG-200413-49
Chestnut et al., 2019 - Zebrafish etv2 knock-in line labels vascular endothelial and blood progenitor cells. Developmental dynamics : an official publication of the American Association of Anatomists   249(2):245-261 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 7 etv2 expression analysis in wild‐type, etv2 ci32G + −/− and etv2 ci32Gt−/− embryos at the 20‐somite, A‐C, and 24 hpf stages, D‐F. Embryos were obtained from an incross of etv2 ci32Gt+/−; UAS:GFP +/+ parents and sorted based on their GFP fluorescence pattern. Antisense etv2 RNA probe which corresponds to the C‐terminal portion of the etv2 coding sequence and 3′UTR downstream of the Gal4 insertion side was used for in situ hybridization (see Experimental Procedures). A‐C, In non‐fluorescent wild‐type siblings (wt), strong etv2 expression is apparent in vascular progenitors in the anterior lateral plate mesoderm, presumptive progenitors of the anterior and common cardinal veins, and in vascular progenitors next to the tailbud (arrows). Weaker expression in the dorsal aorta (DA) is also apparent. Note the reduced etv2 expression in etv2 ci32Gt+/− embryos and nearly absent expression in etv2 ci32Gt−/− embryos. D‐F, In wild‐type siblings, strong etv2 expression is apparent in the cranial venous vasculature, including the primordial midbrain channel (PMBC) and the middle cerebral vein (MCEV), as well as the tail plexus region (arrow). Weaker expression in the DA is also apparent. Note the reduced etv2 expression in etv2 ci32Gt+/− embryos and nearly absent expression in etv2 ci32Gt−/− embryos

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
etsrp WT standard conditions 20-25 somites anterior lateral plate mesoderm ISH
20-25 somites common cardinal vein ISH
20-25 somites vascular cord ISH
Prim-5 common cardinal vein ISH
Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intermediate cell mass of mesoderm ISH
etsrpci32Gt/+; nkuasgfp1aTg standard conditions 20-25 somites anterior lateral plate mesoderm ISH
20-25 somites common cardinal vein ISH
20-25 somites intermediate cell mass of mesoderm ISH
Prim-5 cranial vasculature ISH
Prim-5 dorsal aorta ISH
Prim-5 intermediate cell mass of mesoderm ISH
Prim-5 posterior cardinal vein ISH
etsrpci32Gt/ci32Gt; nkuasgfp1aTg standard conditions 20-25 somites Not Detected anterior lateral plate mesoderm ISH
20-25 somites Not Detected common cardinal vein ISH
20-25 somites intermediate cell mass of mesoderm ISH
Prim-5 Not Detected common cardinal vein ISH
Prim-5 cranial vasculature ISH
Prim-5 Not Detected dorsal aorta ISH
Prim-5 intermediate cell mass of mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
etsrpci32Gt/+; nkuasgfp1aTg standard conditions 20-25 somites anterior lateral plate mesoderm etsrp expression decreased amount, abnormal
20-25 somites common cardinal vein etsrp expression decreased amount, abnormal
20-25 somites intermediate cell mass of mesoderm etsrp expression decreased amount, abnormal
Prim-5 cranial vasculature etsrp expression decreased amount, abnormal
Prim-5 dorsal aorta etsrp expression decreased amount, abnormal
Prim-5 intermediate cell mass of mesoderm etsrp expression decreased amount, abnormal
etsrpci32Gt/ci32Gt; nkuasgfp1aTg standard conditions 20-25 somites anterior lateral plate mesoderm etsrp expression absent, abnormal
20-25 somites common cardinal vein etsrp expression absent, abnormal
20-25 somites intermediate cell mass of mesoderm etsrp expression decreased amount, abnormal
20-25 somites intermediate cell mass of mesoderm etsrp expression decreased distribution, abnormal
Prim-5 common cardinal vein etsrp expression absent, abnormal
Prim-5 cranial vasculature etsrp expression decreased amount, abnormal
Prim-5 cranial vasculature etsrp expression decreased distribution, abnormal
Prim-5 dorsal aorta etsrp expression absent, abnormal
Prim-5 intermediate cell mass of mesoderm etsrp expression decreased amount, abnormal
Prim-5 intermediate cell mass of mesoderm etsrp expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.