FIGURE

Fig 1

ID

ZDB-FIG-200412-8

Publication

Xie et al., 2020 - E2f5 is a versatile transcriptional activator required for spermatogenesis and multiciliated cell differentiation in zebrafish

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Fig 1

Mutation of <italic>e2f5</italic> leads to male infertility.

(A-B) Whole-mount in situ hybridization showing expression of e2f5 at 10-somite stage (10s) and 24 hpf. OP, olfactory placode; OV, otic vesicle; PD, pronephric duct. (C) Genomic structure and sequences of wild- type (wt) and three e2f5 mutant alleles. The underlined sequence in wt indicates PAM sequence of sgRNA target. (D) Phenotypes of male and female wild-type and e2f5 heterozygotes. Only fish exhibiting the male phenotype were present among homozygous mutants. (E) Bar graph showing the percentage of fertilization rates of wild-type, e2f5 homozygote and e2f5 homozygotes carrying Tg(β-actin:gfp-e2f5) transgene as indicated. The numbers of adult males investigated are listed at the bottom. (F) Diagram of the constructs for making gfp-e2f5 transgene. (G-H) H&E staining results showing testes of wild-type (G) and e2f5 homozygous mutants (H). Arrows indicate primary spermatocytes. Asterisks point to mature spermatozoa which were substantially reduced in the mutants. (I-J) Confocal images showing the staining of sperm flagella in wild-type (I) and e2f5 mutant testis (J) visualized with acetylated alpha-tubulin (ac-Tu) antibody. Nuclei and actin filaments were counterstained with DAPI and phalloidin, respectively. Scale bars: 1cm in panel D, 50 μm in panel G, H and 25 μm in panel I, J.

Expression Data

Gene:	e2f5
Fish:	WT
Anatomical Terms:	olfactory placode otic placode otic vesicle pronephric duct spinal cord posterior region
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	e2f5^{ouc2019/ouc2019} e2f5^{ouc2020/ouc2020} e2f5^{ouc2021/ouc2021} e2f5^{ouc2019/ouc2019}; ouc2018Tg e2f5^{ouc2020/ouc2020}; ouc2018Tg e2f5^{ouc2021/ouc2021}; ouc2018Tg (all 6)
Observed In:	female organism female sex determination male organism spermatid spermatocyte whole organism (all 6)
Stage:	Adult

Phenotype Detail

Acknowledgments

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