Fig 5

(A) Schematic of the motility and chemotaxis GOF switches. Table summarizes switch activity and bacterial behaviors +/− aTc. (B) Δmot^GOF or Δche^GOF abundances 24 hpi +/− aTc. Δmot^GOF and Δche^GOF were preinduced overnight in liquid culture prior to inoculation; aTc was maintained in the water for continuous switch activation. Abundances of wild-type Vibrio, Δmot, and Δche in wild-type hosts (from Fig 1A, 24 hpi) are shown for comparison. Bars denote medians and interquartile ranges. Letters denote significant differences. p < 0.05, Kruskal-Wallis and Dunn’s multiple comparisons test. (C) Probability densities showing the spatial distributions of Δmot^GOF and Δche^GOF at 24 hpi. Magenta = uninduced; green = induced. Shaded regions mark standard errors. Sample sizes (populations within individual animals): Δmot^GOF “OFF”, n = 5; Δmot^GOF “ON”, n = 7, Δche^GOF “OFF”, n = 6; Δche^GOF “ON”, n = 6. (D) Maximum intensity projections acquired by LSFM from the same animal showing Δche^GOF undergoing rapid changes in spatial organization following induction. Dashed lines mark approximate intestinal boundary. (E) Abundances of Δmot^GOF and Δche^GOF over time. Magenta and green circles indicate abundances +/− aTc, respectively. Plotted are medians and interquartile ranges (n ≥ 19 animals/marker). Abundances of wild-type Vibrio, Δmot, and Δche (from Fig 1A) are shown for comparison. Significant differences between each mutant and wild-type determined by Mann-Whitney (magenta asterisks: uninduced; green asterisks: induced). ***p < 0.0001. Underlying data plotted in panels B, C, and E are provided in S1 Data. aTc, anhydrotetracycline; GOF, gain-of-function; hpi, hours post induction; LSFM, light sheet fluorescence microscopy; ns, not significant.