FIGURE

Figure 6

ID

ZDB-FIG-200312-12

Publication

Hoelscher et al., 2020 - miR-128a Acts as a Regulator in Cardiac Development by Modulating Differentiation of Cardiac Progenitor Cell Populations

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Figure 6

Role of miR-128a in cardiac development. During murine in vitro differentiation, LNA-mediated miR-128a knockdown in differentiating ES/iPSCs (1) increased cardiac transcription factors such as Isl1, Sfrp5, and Hcn4 but reduced Irx4 at the onset of cardiogenesis; (2) upregulated Isl1-positive CPCs, whereas NkxCE-GFP-positive CPCs were downregulated; and (3) increased the expression of the ventricular cardiomyocyte marker Myl2 accompanied by a reduced beating frequency of early cardiomyocytes. Overexpression of miR-128a (4) diminished the expression of Isl1, Sfrp5, Nkx2.5, and Mef2c, but increased Irx4, (5) enhanced the NkxCE-GFP-positive CPC population, and (6) favored nodal-type-like cardiomyocytes marked by Tnnt2, Myh6, and Shox2 accompanied by increased beating rates. Parts of the figure from BioRender.com

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.