Figure 1

Expression kinetics of HDAC transcripts in primary human macrophages following Mtb infection. Mϕ1 and Mϕ2 derived from 4 different donors were mock infected or infected with Mtb for 1 h at MOI 10. Transcript levels of HDAC1-11 were determined in triplicate using qRT-PCR before (0 h baseline) and 4 and 24 h post-infection. Data was normalized to GAPDH (ΔCt) and mean expression levels of triplicate samples were calculated for each donor. (A) Heatmap displaying median log₂ fold changes (FC) of HDAC1-11 expression levels (pooled data of 4 donors) in response to Mtb infection compared to their respective baseline controls. (B) Dot plots displaying log₂ FC expression levels of HDAC1, 3, 5, 7, 10, and 11 in response to Mtb infection compared to their respective baseline controls, calculated using the 2^−ΔΔCT formula. Each dot represents a single donor. Horizontal lines indicate median log₂ FC values of all 4 donors and whiskers represent 95% confidence intervals. Statistically significant differences compared to uninfected controls were tested using a paired sample t-test (*p < 0.05, **p < 0.01).