Figure 5

Engineering a MF. A) Left: TEM micrograph of AOs based on PMOXA₆‐PDMS₄₄‐PMOXA₆ polymersomes loaded with horseradish peroxidase (HRP) and equipped with OmpF. Scale bar: 500 nm. Right: Amplex UltraRed conversion kinetics of AOs equipped with OmpF (red) and AOs without OmpF (blue). Standard deviations are based on 3 individual measurements. B) Spatial colocalization in E‐GPMVs. Left: Schematic representation of E‐GPMVs containing SRB‐loaded polymersomes and cytosolic GFP protein. Middle: CLSM micrograph of E‐GPMVs containing SRB‐loaded polymersomes (Red signal) and cytosolic GFP protein (Green signal). Right: Colocalization analysis of SRB‐loaded polymersomes and GFP protein within E‐GPMVs. PCC = 0.16 ± 0.14, M1 = 0.35 ± 0.1, M2 = 0.09 ± 0.09. n = 5 independent CLSM images. C) Functional MFs containing AOs, which convert in situ Amplex UltraRed into resorufin‐like product. Left: Schematic representation of MFs containing AOs. Middle: CLSM micrograph of real‐time production of the resorufin‐like product by AOs inside MFs (Red signal). Right: Relative fluorescence intensity recordings of individual MFs equipped with AOs. See Movie S9, Panel (D) in the Supporting Information) Control MFs equipped with AOs with no OmpF. Left: Schematic representation of E‐GPMVs containing AOs without OmpF. Middle: CLSM Micrograph demonstrating the absence of conversion of Amplex UltraRed into the resorufin‐like product when AOs inside MFs have no OmpF. Right: Relative fluorescence intensity recordings of individual MFs loaded with AOs without OmpF; See Movie S10 in the Supporting Information.