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Macrophages lacking Cxcr3.3 efficiently clear IC bacteria. Cxcr3.3‐deficient larvae and their WT siblings were infected in the BI at 28 hpf with 200 CFU of the ΔERP M. marinum‐wasabi strain that is unable to survive and replicate inside acidic compartments and can be easily cleared by macrophages. The total number of bacterial clusters in every fish was quantified (A). We divided the bacterial clusters into 3 groups based on the number of bacteria they contained (1–5, 1–6, and >10) to assess bacterial clearance at 44 hpi (B). No difference between WT and cxcr3.3–/– cluster size distributions (frequency in %) was found (C). A Mann‐Whitney test was conducted to analyze the overall bacterial burden of the pooled data of 3 independent replicates of 9 fish each. Data are shown as mean ± sem (A). A Kolmogorov‐Smirnov test was used to analyze the distribution of bacterial cluster sizes (C) (ns P > 0.05)
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