FIGURE

Figure 5.

ID

ZDB-FIG-200208-28

Publication

Honkoop et al., 2019 - Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart

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Figure 5.

2-Deoxy glucose impairs cardiomyocyte proliferation.

(a) Experimental design for the 2-DG injections to inhibit glycolysis in injured zebrafish hearts. (b) Confocal image of injured zebrafish hearts at seven dpi either injected with PBS or 2-DG stained for Mef2c (green) and PCNA (magenta). Zoom-in images of the borderzone are shown below overview pictures for both PBS (cyan box) and 2-DG (orange box). Arrowheads indicate nuclei positive for Mef2c and PCNA. Scale bar indicates 200 μm (overview) or 20 μm (zoom-in). (c) Quantification of the proliferating cardiomyocytes (double Mef2c/PCNA positive) in the border zone of PBS and 2-DG treated hearts represented as the proportion of proliferating cardiomyocytes compared to the average percentage in the PBS injected group. Each dot represents a single heart (three sections per heart analyzed). Hearts were pooled from two separate experiments. Error bars represent mean ± standard deviation. ***, p<0,0001.

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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