FIGURE

Figure 3

ID

ZDB-FIG-200125-89

Publication

Wang et al., 2019 - Toxic Effects of Paclobutrazol on Developing Organs at Different Exposure Times in Zebrafish

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Figure 3

Pharyngeal arch development is affected according to the initial PBZ exposure stage in zebrafish embryos. Zebrafish embryos were exposed to a range of PBZ concentrations (0.34, 3.4, and 17 μM) beginning at different stages (24, 36, 48, 60, 72, and 96 hpf). The head skeleton of 5 dpf fish was analyzed by Alcian blue staining. Pharyngeal arch phenotypes were categorized as (A) ‘Normal’, ‘Mild’ defects or ‘Severe’ defects. Images were taken from the dorsal view. (B) Frequency of pharyngeal arch phenotypes was compared in zebrafish embryos treated with different concentrations of PBZ at different exposure stages. Data represent the mean of three independent experiments; 30 embryos were assessed in each treatment (N = 3. n = 90 embryos).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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