Figure 3
Pharyngeal arch development is affected according to the initial PBZ exposure stage in zebrafish embryos. Zebrafish embryos were exposed to a range of PBZ concentrations (0.34, 3.4, and 17 μM) beginning at different stages (24, 36, 48, 60, 72, and 96 hpf). The head skeleton of 5 dpf fish was analyzed by Alcian blue staining. Pharyngeal arch phenotypes were categorized as (