FIGURE

Figure 5

ID

ZDB-FIG-200125-91

Publication

Wang et al., 2019 - Toxic Effects of Paclobutrazol on Developing Organs at Different Exposure Times in Zebrafish

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Figure 5

Liver defects are more severe when exposure to PBZ begins at early stages. Zebrafish embryos were exposed to a range of PBZ concentrations (0.34, 3.4, and 17 μM) beginning at different stages (24, 36, 48, 60, 72, and 96 hpf). Embryos were collected at 5 dpf and analyzed by whole-mount in situ hybridization with an lfpb antisense riboprobe; lfpb is specifically expressed in liver. The liver phenotype was categorized as (A) ‘Normal’, ‘Mild’ or ‘Severe’ defects. Images were taken from ventral view. (B) Frequency of liver phenotypes was compared in zebrafish embryos treated with different concentrations of PBZ at different exposure stages. Data represent the mean of three independent experiments; 30 embryos were assessed in each treatment (N = 3. n = 90 embryos).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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