Radiation specifically damages the brain capillaries of transgenic zebrafish. (A) A schematic representation of X-ray radiation for transgenic zebrafish at 0, 5 and 10 Gy respectively. (B) The ability of maintain stability over 15 s (top) and moving tracks of zebrafish over 1 minute (bottom) at 2-day post radiation in 0, 5 and 10 Gy group (n = 3 zebrafishes per group). (C) Live example of images (each representative of 6 zebrafishes) showing the midbrain region of the zebrafish larva, and depicting endothelial cells (green) of cerebral capillaries in 0, 5 and 10 Gy group at 4-day post radiation. The right images are magnifications of the boxed areas in left images. Scale bars, 100 µm. (D) Morphometric analyses of cerebral capillaries diameter in 0, 5 and 10 Gy group at 0 day, 2-day, 4-day post radiation respectively (n = 6 zebrafishes per group). (E) The survival analysis of zebrafishes in 0, 5 and 10 Gy group (n = 10 zebrafishes per group). (F) Live example of images (each representative of 6 zebrafishes) depicting systole and diastole of heart (red) in 0 and 10 Gy group at 2-day post radiation (left), and heart rate of zebrafishes per minute in control, 5 and 10 Gy group at 2-day post radiation (right). Statistical analysis in D and F was performed using t-test: **P < 0.05, ***P < 0.01. Data represent the mean ± s.e.m.
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