ZFIN Image: Chagovetz et al., 2019, Fig. 2.

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Figure Caption

Fig. 2.

Formation of Shh-dependent muscle in ryr mutants. Muscle cell type patterning in wild-type and mutant zebrafish 24 hpf embryos was assessed by immunohistochemical staining for expression of the Prox1 and Engrailed nuclear proteins. (A-D) Representative images of Prox1 (magenta) and Engrailed (green) staining of somitic muscle in wild-type (A), MZryr1a (B), MZryr3 (C) and MZryr1a;MZryr3 (D) embryos. Slow muscle pioneer cells (MPs) were identified as cells that expressed both Prox1 and Engrailed antigens; medial fast fibers (MFFs) were identified as cells that expressed only the Engrailed antigen; and superficial slow fibers (SSFs) were identified as cells that expressed only the Prox1 antigen. (E) Quantification of Shh-dependent muscle cell types. One-way ANOVA was used to determine statistical relationships with Sidak's multiple comparisons test used to adjust P-values. n.s., not significant; *P<0.01, **P<0.001.

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Acknowledgments

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