FIGURE

Figure 2

ID

ZDB-FIG-191230-887

Publication

Cheng et al., 2019 - Ciglitazone-a human PPARγ agonist-disrupts dorsoventral patterning in zebrafish

Other Figures

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Figure 2

Knockdown of pparγ adversely affects development within the first 96 h.

Abundance of pparγ mRNA within whole zebrafish embryos from 0.75 to 96 hpf (A). Confirmation of knockdown by 24 hpf following injection of splice-blocking pparγ-MOs. Lanes 1–3: 6-hpf embryos injected with nc-MO; lanes 4–6: 6-hpf embryos injected with pparγ-MO; lanes 7–9: 24-hpf embryos injected with nc-MO; and lanes 10–12: 24-hpf embryos injected with pparγ-MO (B). Representative images of water-, nc-MO-, or pparγ-MO-injected embryos from 6 to 96 hpf before and after staining with Oil Red O (ORO) (C–FF). Arrows point to mild dorsoventral patterning defects (ventralization), whereas arrowheads point to cardiac edema and cardiac looping defects.

Expression Data

Gene:	pparg
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	2-cell to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-pparg
Knockdown Reagent:	MO3-pparg
Observed In:	heart heart looping whole organism
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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