Fig 1

(A) Lethality of solvent ethanol to zebrafish after exposure of 24, 48 and 72h at different ethanol concentrations. The experiment was repeated for three times, with 30 zebrafish larvae in each group. Baseline adjusted values (mean±SD) are presented. *P<0.05,**P<0.01 versus control. (B) Influence of solvent ethanol to the morphology of zebrafish larvae after exposure for 24 h (a, b, c, d, e, f and g), 48 h (a’, b’, c’, d’, e’, f’ and g’) and 72 h(a”, b”, c”, d”, e”, f” and g”) at blank (a, a’ and a”) and at the ethanol concentrations of 0.6 mM(b, b’ and b”), 0.8 mM(c, c’ and c”), 1.0 mM(d, d’ and d”), 1.2 mM(e, e’ and e”), 1.5 mM(f, f’ and f”) and 1.8 mM(g, g’ and g”). Swim bladder reduction of zebrafish larvae was pointed out by the white arrow, yolk sac edema was pointed out by the red arrow, and pericardium edema was pointed out by the black arrow.