FIGURE

Fig. 3

ID
ZDB-FIG-191230-20
Publication
Hu et al., 2019 - Infection and RNA-seq analysis of a zebrafish tlr2 mutant shows a broad function of this toll-like receptor in transcriptional and metabolic control and defense to Mycobacterium marinum infection
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Fig. 3

Quantification of bacterial burden and survival after M. marinum infection. tlr2+/+ (a), tlr2+/− (b) and tlr2−/− (c) embryos were infected with mCherry-labeled M. marinum strain Mma20 at a dose of ~ 150 CFU by caudal vein infection at 28 hpf. Representative images for bacterial pixel count in tlr2+/+ (a), tlr2+/− (b) and tlr2−/− (c) were taken at 4 dpi. Bacterial burden of tlr2+/+, tlr2+/− and tlr2−/− were also quantified at 3 dpi (d) and 4 dpi (e). Bacterial burdens were quantified by using bacterial fluorescence pixels. Red stars in (e) indicate the data for the representative images shown in (a-c). In (d, e), data (mean ± SEM) were combined from two independent experiments. Statistical significance of differences was determined by one-way ANOVA with Tukey’s Multiple Comparison method as a post-hoc test for comparison between more than two groups (d, e). Percent of survival curves for tlr2+/+ (n = 47), tlr2+/− (n = 49) and tlr2−/− (n = 49) (f) are based on two independent experiments. Statistical significance of difference was determined by a log-rank (Mantel-Cox) test. ns, non-significant; *, P < 0.05, **, P < 0.01, ****, P < 0.0001

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
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