Figure 10

BrdU immunohistochemistry analysis of the effects of embelin in improving neurogenesis and migration of cells generated during adulthood from the molecular layer to the granular layer within the molecular zone of the hypothalamus, the internal cellular layer of the olfactory bulb of the periventricular gray zone of TeO, and the medial zone of the dorsal telencephalic area against pentylenetetrazole-induced epilepsy the zebrafish brain. (I) BrdU-positive staining revealed labeling and number of migration of cells at day 15 of maturation and differentiation stage at the molecular zone of each section of zebrafish brain with BrdU labeling. Photomicrographs of the sagittal section of treatment groups were (A) control, (B) PTZ 170 mg/kg alone, (C) EMB 0.156 mg/kg + PTZ, and (D) EMB 0.625 mg/kg + PTZ. Representative photomicrographs were taken at magnifications of 40X and 100X. (II) Quantification of BrdU population: Data are expressed as mean ± SEM, n = 6, and statistical analysis by one-way ANOVA followed by Dunnett’s test *P < 0.05, **P < 0.01, and ***P < 0.001.