Figure 9

(A) BrdU immunohistochemistry analysis of the protective effects of embelin at the proliferation (day 0) and survival (day 15) stage against pentylenetetrazole-induced epilepsy the zebrafish brain. (A I) BrdU-positive staining revealed labeling of mitotic cells of immature neurons in the subgranular zone of the periventricular gray zone of tectum opticum with BrdU. Photomicrographs of the sagittal section of treatment groups were (A) control, (B) PTZ 170 mg/kg, (C) EMB 0.156 mg/kg + PTZ, and (D) EMB 0.625 mg/kg + PTZ. Representative photomicrographs were taken at magnifications of 40X and 200X. (II) Quantification of BrdU positive cells. Data are expressed as means mean ± SEM, n = 6, and statistical analysis by one-way ANOVA followed by Dunnett’s test ^∗∗P < 0.01, and ***P < 0.001. (A II) BrdU immunohistochemistry analysis of the effects of embelin in improving neurogenesis and migration of cells generated during 15 days from the molecular layer to the granular layer within the dorsal zone of the periventricular hypothalamus against pentylenetetrazole-induced epilepsy the zebrafish brain. (I) BrdU-positive staining revealed labeling and migration of cells at day 15 of cell maturation and differentiation stage within the valvula cerebelli zone of cerebellum with BrdU labeling. Photomicrographs of the sagittal section of treatment groups were (A) control, (B) PTZ 170 mg/kg alone, (C) EMB 0.156 mg/kg + PTZ, and (D) EMB 0.625 mg/kg + PTZ. Representative photomicrographs were taken at magnifications of 40X and 100X. (II) Quantification of BrdU population: Data are expressed as mean ± SEM, n = 6, and statistical analysis by one-way ANOVA followed by Dunnett’s test ^∗∗P < 0.01, and ^∗∗∗P < 0.001. (B) Graphical representation of cell migration in zebrafish brain. (I) represents location of positive BrdU cells at day 0. (II) represents migration and location of positive BrdU cells at day 15.