FIGURE

Fig 6

ID

ZDB-FIG-191230-1162

Publication

Lischik et al., 2019 - Enhanced in vivo-imaging in medaka by optimized anaesthesia, fluorescent protein selection and removal of pigmentation

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Fig 6

The established toolbox enables imaging of previously challenging organs and tissues, such as the retina and the brain.

Using the pigment double knockout in combination with α-Bungarotoxin, eGFP and mCherry coupled to histone2a the imaging was conducted in a single plane illumination microscope (SPIM) we could image the head region of wild-type and spooky embryos. Hollow arrowheads point towards the eyes in A and B. (A) Maximum projection of H2A-mCherry signal of a lateral view of a wild-type medaka embryo. The eye is not penetrable and no nuclei are visible within or behind it. (A') Maximum projection of H2A-mCherry signal of a lateral view of a spooky medaka embryo. The eye is penetrable and nuclei are visible within or behind it. (B) Stitched maximum projections of a dorsal view of a spooky embryo. This embryo was imaged for 48 hours, which can be seen in S3 Movie.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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