FIGURE

Fig. 4

ID

ZDB-FIG-191219-3

Publication

Jin et al., 2019 - Dysregulation of Microglial Function Contributes to Neuronal Impairment in Mcoln1a-Deficient Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

The T519 to I Mutation Reduces Mcoln1a-Mediated Ca²⁺ Efflux

(A) Representative calcium efflux of WT (blue) and mutant Mcoln1a channel. Stars represent calcium peaks we quantified.

(B) Quantification of the mean of the peak of ΔF/F0 (representing Trpml1a-mediated Ca²⁺efflux) in the macrophages in WT embryos injected with WT CMV:GCaMP6s-mcoln1a construct (blue dots) and the macrophages in biluo mutants injected with mutant CMV:GCaMP6s-mcoln1aT519I (red triangles). ΔF/F0 is calculated as (F-F0)/F0, where F0 is the baseline fluorescence signal. Error bars represent mean ± SD. n(WT late endosome) = 20 from 8 macrophages, n(biluo late endosome) = 20 form 7 macrophages.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mcoln1a^hkz13/hkz13
Observed In:	microglial cell vacuolar calcium ion homeostasis
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ iScience