ZFIN Image: Jin et al., 2019, Fig. 4

Image

Figure Caption

Fig. 4 The T519 to I Mutation Reduces Mcoln1a-Mediated Ca²⁺ Efflux

(A) Representative calcium efflux of WT (blue) and mutant Mcoln1a channel. Stars represent calcium peaks we quantified.

(B) Quantification of the mean of the peak of ΔF/F0 (representing Trpml1a-mediated Ca²⁺efflux) in the macrophages in WT embryos injected with WT CMV:GCaMP6s-mcoln1a construct (blue dots) and the macrophages in biluo mutants injected with mutant CMV:GCaMP6s-mcoln1aT519I (red triangles). ΔF/F0 is calculated as (F-F0)/F0, where F0 is the baseline fluorescence signal. Error bars represent mean ± SD. n(WT late endosome) = 20 from 8 macrophages, n(biluo late endosome) = 20 form 7 macrophages.

Figure Data

Phenotype details

Acknowledgments

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