FIGURE

Fig. S5

ID

ZDB-FIG-190822-29

Publication

Pei et al., 2018 - Guided genetic screen to identify genes essential in the regeneration of hair cells and other tissues

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Fig. S5

Hair cell development in the regeneration-specific mutants and the expression patterns of the identified genes.

(A-E) Quantification of hair cell development in the mutant embryos carrying the rnpc3^la028632 retroviral insertional mutation (A), hspe1^hg76 (6 bp deletion) mutation (B), hspa13^hg78 (12 bp deletion) mutation (C), smn1^fh299 (ENU Y262X) mutation (D), and gemin5^hg80 (1 bp deletion) mutation (E). Approximately 10 embryos were analyzed for each data point. The graphs show the mean and s.e.m. The difference between the wild-types and homozygous mutants was not significant (n. s.) for all the mutations, although there was a modest reduction trend in hspa13. (F-J) Whole-mount in situ analysis of the expression of the regeneration genes in TAB-5 wild-type embryos at 4 dpf. Arrows in F-J point to the expression in liver. Arrowheads in J point to the expression of rnpc3 in lateral line neuromasts.

Expression Data

Genes:	gemin5 hspa13 hspe1 rnpc3 smn1
Fish:	WT
Anatomical Terms:	liver posterior lateral line neuromast
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gemin5^hg80/hg80 gemin5^hg81/hg81 hspa13^hg78/hg78 hspa13^hg79/hg79 hspe1^hg76/hg76 hspe1^hg77/hg77 rnpc3^{la028632Tg/la028632Tg} smn1^fh229/fh229
Observed In:	neuromast development neuromast hair cell posterior lateral line neuromast
Stage:	Day 4

Phenotype Detail

Acknowledgments

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