FIGURE

Fig. 3

ID

ZDB-FIG-190814-20

Publication

Pei et al., 2018 - Guided genetic screen to identify genes essential in the regeneration of hair cells and other tissues

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Fig. 3

Mutations specifically affecting hair cell regeneration. a A fluorescent image of a zebrafish embryo at 5 dpf stained with Yopro-1, showing the distribution of neuromast hair cells. Arrows point to the P1–P4 neuromasts that were used for hair cell counting. b A magnified image of neuromast hair cells in the P1 neuromast. c–g Quantification of impaired hair cell regeneration caused by rnpc3^la028632 retroviral insertional mutation (c), hspe1^hg77 (6 bp CRISPR deletion) mutation (d), hspa13^hg78 (12 bp CRISPR deletion) mutation (e), smn1^fh299 (ENU missense Y262X) mutation (f), and gemin5^hg80(1 bp CRISPR deletion) mutation (g). The graphs are obtained from analyzing and then genotyping ~45 embryos generated from a pairwise incross of heterozygotes. Graphs show the mean and s.e.m. The difference between the wild-types and homozygotes is significant for all mutations (p < 0.05)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gemin5^hg80/hg80 gemin5^hg81/hg81 hspa13^hg78/hg78 hspa13^hg79/hg79 hspe1^hg76/hg76 hspe1^hg77/hg77 rnpc3^{la028632Tg/la028632Tg} smn1^fh229/fh229
Condition:	chemical ablation: neuromast hair cell, chemical treatment by environment: copper(II) sulfate
Observed In:	neuromast hair cell neuromast hair cell regeneration posterior lateral line neuromast
Stage:	Day 5

Phenotype Detail

Acknowledgments

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