Fig. 3

Henna and Lawsone activate AhR in zebrafish larvae. (A,B) Fold induction of CYP1A, AhRRa and AhRRb transcripts from zebrafish larvae (2 days post-fertilization, dpf) treated (red squares) or not (black circles) for 2 h with 5 µM of AhR inhibitor CH223191, followed by further 4 h stimulation with (A) Henna (equivalent to 10 μM Lawsone), (B) Lawsone (10 μM) or DMSO vehicle control. Triplicates of 12 larvae depicted in each data point. (C) Scheme of the semi-high throughput experimental design developed to measure zebrafish larvae CYP1A enzymatic activity. (D) Representative images obtained upon CYP1A activity measurements using an Array Scan TM XTI Live High Content Platform. (E) CYP1A enzymatic activity expressed as total intensity of resorufin detected per larva (each dot represents one larva). 1 representative experiment out of 3 are shown (n = 36 larvae per condition). (A,B) Data from 1 representative experiment out of 3 is shown. (A,B) Floating bars, Mean Min to Max. (A,B) Two-way ANOVA with Bonferroni’s test. (E) Two-way ANOVA with Fisher’s test. **P < 0.01, ***P < 0.001; ****P < 0.0001.