FIGURE

Figure S3

ID

ZDB-FIG-190806-16

Publication

Charlton-Perkins et al., 2019 - Genetic control of cellular morphogenesis in Müller glia

Other Figures

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Figure S3

Phenotypes of conserved highly conserved MG cell genes. (a) Schematic representation of how highly conserved genes we bioinformatically identified. (b) slc45a5 controls have no observable MG phenotype. (b) timp2b mutants have defects in cell body position. (c) nphs1 mutants have defects in ILM, IPL, and tiling. (d) kirrela mutants have defects in cell body position and tiling. (e) wt1 mutants have defects in cell body position, IPL, OPL, and tiling. (f) mmp2 mutants have defects in OLM, ILM, and tiling. (g) cadm4 mutants have defects in OPL, IPL, ILM, and tiling. (h) Cadm1a mutants have defects in IPL and tiling. (i) cadm2b mutants have defects in a cell body positing, IPL OPL and tiling. (j–l) Percentages of individual phenotypes observed in all animals from this screen. Dashed lines represent levels of significance from Fisher's exact test after Boniforni multiple test correction (bottom = p < 0.05, top = p < 0.001). Scale bars = 8μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi2001Tg + CRISPR1-cadm2b + CRISPR2-cadm2b mi2001Tg + CRISPR1-cadm4 + CRISPR2-cadm4 mi2001Tg + CRISPR1-kirrel1a + CRISPR2-kirrel1a mi2001Tg + CRISPR1-nphs1 + CRISPR2-nphs1 mi2001Tg + CRISPR2-cadm1a + CRISPR3-cadm1a mi2001Tg + CRISPR2-timp2b + CRISPR3-timp2b mi2001Tg + CRISPR3-wt1b + CRISPR4-wt1b mi2001Tg + CRISPR5-mmp2 + CRISPR6-mmp2
Knockdown Reagents:	CRISPR1-cadm2b CRISPR1-cadm4 CRISPR1-kirrel1a CRISPR1-nphs1 CRISPR2-cadm1a CRISPR2-cadm2b CRISPR2-cadm4 CRISPR2-kirrel1a CRISPR2-nphs1 CRISPR2-timp2b CRISPR3-cadm1a CRISPR3-timp2b CRISPR3-wt1b CRISPR4-wt1b CRISPR5-mmp2 CRISPR6-mmp2
Observed In:	inner limiting membrane Muller cell Muller cell cell body outer limiting membrane retinal inner plexiform layer retinal outer plexiform layer
Stage:	Day 5

Phenotype Detail

Acknowledgments

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