FIGURE

Figure S1

ID

ZDB-FIG-190806-14

Publication

Charlton-Perkins et al., 2019 - Genetic control of cellular morphogenesis in Müller glia

Other Figures

All Figure Page

Back to All Figure Page

Figure S1

Phenotypes of gene mutants enriched over windows of MG cell differentiation. (a) slc45a5 controls have no observable MG phenotype. (b) f8 mutants have defects in cell body position, OLM, ILM, IPL, OPL, and tiling. (c) cdhr1 mutants have defects in cell body position, OLM, ILM, IPL, OPL, and tiling. (d) sptbn mutants have defects in OLM, IPL, OPL, and tiling. (e) mapa1b mutants have defects in OLM, IPL, and OPL. (f) xirp1 mutants have defects in OPL and tiling. (g) lamb2 mutants have defects in ILM, IPL, and tiling. (h) Cadm1b mutants have defects in ILM, IPL, OPL, and tiling. (i) sox10 mutants have defects in IPL and tiling. (j) nfat5 mutants have tiling defects. (k) snx19a mutants have tiling defects. (l) Percentages of individual phenotypes observed in all animals from this screen. Dashed lines represent levels of significance from Fisher's exact test after Boniforni multiple test correction (bottom = p < 0.05, top = p < 0.001). Scale bars = 8μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi2001Tg + CRISPR1-cadm1b + CRISPR2-cadm1b mi2001Tg + CRISPR1-cdhr1a + CRISPR2-cdhr1a mi2001Tg + CRISPR1-f8 + CRISPR2-f8 mi2001Tg + CRISPR1-lamb2 + CRISPR2-lamb2 mi2001Tg + CRISPR1-nfat5b + CRISPR2-nfat5b mi2001Tg + CRISPR1-snx19a + CRISPR2-snx19a mi2001Tg + CRISPR1-sptbn5 + CRISPR2-sptbn5 mi2001Tg + CRISPR3-sox10 + CRISPR4-sox10 mi2001Tg + CRISPR3-xirp1 + CRISPR4-xirp1
Knockdown Reagents:	CRISPR1-cadm1b CRISPR1-cdhr1a CRISPR1-f8 CRISPR1-lamb2 CRISPR1-nfat5b CRISPR1-snx19a CRISPR1-sptbn5 CRISPR2-cadm1b CRISPR2-cdhr1a CRISPR2-f8 CRISPR2-lamb2 CRISPR2-nfat5b CRISPR2-snx19a CRISPR2-sptbn5 CRISPR3-sox10 CRISPR3-xirp1 CRISPR4-sox10 CRISPR4-xirp1
Observed In:	inner limiting membrane Muller cell Muller cell cell body outer limiting membrane retinal inner plexiform layer retinal neural layer retinal outer plexiform layer
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Glia