FIGURE

Figure 3

ID

ZDB-FIG-190723-1192

Publication

Charlton-Perkins et al., 2019 - Genetic control of cellular morphogenesis in Müller glia

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

Discrete gene expression regulates MG cell compartment morphology. (a) Heatmap to show the relative gene expression for genes tested. These were all screen in F0 CRISPR injected mutants. (b) Summary of phenotypes observed for genes enriched across windows of MG differentiation. Red—phenotype, blue—no‐phenotype. (c) lamb4 mutants have defects in the apico‐basal distribution of MG cell bodies only. (d) dcaf8 mutants have defects in the OLM and OPL. (e) mmp28 CRISPR injected mutants have defects in the ILM only. (f) egr1 mutants have defects in the IPL and OPL. (g) slitkr2 mutants have defects in the OPL layer only. (h) icn2 mutants have tiling defects only. 1. Frequency (%) of phenotypes observed in each MG compartment in F0 CRISPR screen 2. Scale bars = 8 μm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi2001Tg + CRISPR1-dcaf8 + CRISPR2-dcaf8 mi2001Tg + CRISPR1-egr1 + CRISPR2-egr1 mi2001Tg + CRISPR1-icn2 + CRISPR2-icn2 mi2001Tg + CRISPR1-lamb4 + CRISPR2-lamb4 mi2001Tg + CRISPR1-mmp28 + CRISPR2-mmp28 mi2001Tg + CRISPR1-slitrk2 + CRISPR2-slitrk2
Knockdown Reagents:	CRISPR1-dcaf8 CRISPR1-egr1 CRISPR1-icn2 CRISPR1-lamb4 CRISPR1-mmp28 CRISPR1-slitrk2 CRISPR2-dcaf8 CRISPR2-egr1 CRISPR2-icn2 CRISPR2-lamb4 CRISPR2-mmp28 CRISPR2-slitrk2
Observed In:	inner limiting membrane Muller cell Muller cell cell body outer limiting membrane retinal inner plexiform layer retinal outer plexiform layer
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Glia