FIGURE

Fig. 2

ID

ZDB-FIG-190725-86

Publication

Viau et al., 2018 - Cilia-localized LKB1 regulates chemokine signaling, macrophage recruitment, and tissue homeostasis in the kidney

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Fig. 2

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Fig. 2

LKB1 interacts with ANKS3, NEK7, and NPHP1

LKB1 interactors identified by a proteomic screen in mouse kidney medulla. See also Datasets EV1 and EV2.
Immunoprecipitates (IP) from mouse kidney medulla. ANKS3 and NEK7 are enriched in the LKB1 precipitates. Representative Western blot of three independent experiments. Expected molecular weights: ANKS3 (72 kDa), LKB1 (54 kDa), and NEK7 (35 kDa).
Representative confocal microscopy images of primary cilia (acetylated tubulin expressing, Ac‐Tub) and ANKS3 in MDCK cells expressing inducible shRNA against Anks3 (Anks3‐i1) after tetracycline induction (Tet). Scale bar: 5 μm. See also Appendix Fig S3F.
Quantification of ANKS3‐positive primary cilia in Anks3‐i1 MDCK cells. Blinded quantification of five fields of view per biological replicate.
Confocal microscopy images of collecting duct (AQP2 expressing) from 5‐week‐old wild‐type mice. Anti‐ANKS3 antibody (upper panel), but not control IgG (lower panel), detects a signal in cilia (Ac‐Tub). Representative images of n = 5 mice. Scale bar: 5 μm.
IP from HEK 293T cells. Endogenous LKB1 is enriched in the precipitates of FLAG‐NPHP1. Representative Western blot of three independent experiments. See also Fig EV2A–C.
Zebrafish embryos injected with control morpholino (MO) or MO targeting nphp1at 48 h post‐fertilization. Arrowheads indicate pronephric cysts. Scale bar: 100 μm.
Quantification of embryos with pronephric cysts. Numbers in bars represent n analyzed embryos.

Data information: In (D), each circle represents one biological replicate. Bars indicate mean. Paired t‐test, *P < 0.05. In (H) mean ± SD. ANOVA followed by the Tukey–Kramer test, *P < 0.05, **P < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	li1Tg + MO1-nphp1 + MO4-tp53 li1Tg + MO3-stk11 + MO4-tp53 li1Tg + MO1-nphp1 + MO3-stk11 + MO4-tp53
Knockdown Reagents:	MO1-nphp1 MO3-stk11 MO4-tp53
Observed In:	pronephros
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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