FIGURE

Fig. 5

ID

ZDB-FIG-190723-2632

Publication

Heston et al., 2017 - To transduce a zebra finch: interrogating behavioral mechanisms in a model system for speech

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Fig. 5

Characterization of four AAVs obtained from the U Penn viral vector core for their ability to transduce Area X neurons. In all panels, green signals show GFP, red signals show NeuN, and blue signals show DAPI staining. aLeft photomicrograph reveals that AAV2/5 transfects large portions of the brain except for Area X. Scale bar 1000 μm. Right higher magnification shows robust transduction in the striatopallidum outlying Area X. Scale bar 50 μm. b AAV2/1 shows robust transduction but also induces lesions at the injection site. Scale bar 500 μm. c Representative photomicrograph shows that AAV2/rh10 has a low transfection rate in Area X and induces a lesion at the injection site. Scale bar 500 μm. dRight photomicrograph shows low levels of transduction by AAV2/8. Scale bar 500 μm. Even at higher magnification (right), there were very few GFP-positive cell bodies in the most densely transfected region. Scale bar 100 μm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Physiol. A