Characterization of three lentiviruses obtained from the UCLA viral vector core for their ability to transduce Area X neurons. aTop control (left) and experimental (right) constructs are depicted. Below photomicrographs show GFP expression levels following injection into Area X. These viruses achieved sparse GFP expression that could only be detected using a GFP antibody in sections that received the control construct (e.g., left panel). Little to no GFP was observed in the contralateral hemisphere injected with the experimental virus, despite immunological enhancement with an anti-GFP antibody (right panel). Scale bar 100 uM. b Photomicrographs of Area X from a bird brain injected with ultra-centrifuged hSyn1 lentivirus. GFP is shown as green. The tissue was counterstained for NeuN (red) and DAPI (blue). No fluorescence was detected without an anti-GFP antibody. Top left image shows GFP positive signal following conventional immunohistochemical enhancement anti-GFP antibody. A higher magnification of the same section (top right). Bottom images show enhancement of GFP signal following TSA amplification. Despite enhancement, the infection was sparse with few GFP-positive cell bodies. c Photomicrograph shows lesion site in Area X following injection of lentivirus containing the PGK promoter. Scale bar 100 μm
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