Figure 4

Quantification of proliferating cardiomyocytes in cryoinjured hearts. Representative images at 7 dpi of PCNA, mef-2, DAPI, merged image and inset of merged image in scramble (A–D,D’) and in let-7c antagomir hearts (E–H,H’). Representative images at 28 dpi of BRDU, MHC, DAPI, merged image and inset of merged image in scramble (I–L,L’) and in let-7c antagomir hearts (M–P,P’). Positive cardiomyocytes marked with arrowheads. In PCNA images (A–H’) mef-2 marks cardiomyocytes in red, PCNA positive cells marked in green and DAPI nuclei marked in blue. In BRDU images (I–P’), MHC marks myocardium in green, BRDU positive cells marked in red and DAPI nuclei marked in blue. Insets are magnified images of merged image (D’,H’,L’,P’). Newly formed cardiomyocytes are quantified by PCNA staining at 7 dpi and 14 dpi (Q) and by BRDU labeling at 14 dpi and 28 dpi (R). (PCNA: n = 3 for 7 dpi antagomir and scramble, n = 8 for 14 dpi antagomir and scramble. BRDU: n = 10 for 14 dpi antagomir, n = 9 for 14 dpi scramble, n = 8 for 28 dpi antagomir and n = 6 for 28 dpi scramble). Scale bar: 100 μm. * p ≤ 0.05.