PUBLICATION
Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish
- Authors
- Narumanchi, S., Kalervo, K., Perttunen, S., Wang, H., Immonen, K., Kosonen, R., Laine, M., Ruskoaho, H., Tikkanen, I., Lakkisto, P., Paavola, J.
- ID
- ZDB-PUB-190418-3
- Date
- 2019
- Source
- Journal of cardiovascular development and disease 6(2): (Journal)
- Registered Authors
- Keywords
- cardiac regeneration, epicardial cells, fibrin, let-7c, miRNAs, proliferating cardiomyocytes
- MeSH Terms
- none
- PubMed
- 30987331 Full text @ J Cardiovasc Dev Dis
Citation
Narumanchi, S., Kalervo, K., Perttunen, S., Wang, H., Immonen, K., Kosonen, R., Laine, M., Ruskoaho, H., Tikkanen, I., Lakkisto, P., Paavola, J. (2019) Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish. Journal of cardiovascular development and disease. 6(2):.
Abstract
The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker raldh2 at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c. These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping