FIGURE

Figure 5

ID

ZDB-FIG-190723-1817

Publication

Liu et al., 2014 - Functional Variants in DPYSL2 Sequence Increase Schizophrenia Risk and Suggest a Link to mTOR Signaling

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Figure 5

(A) Representative polysome profile of the 260-nm ultraviolet absorption through the sucrose gradient. Cytoplasmic extracts from HEK293 cells transfected with DNR luciferase constructs were layered over a 15–50% sucrose gradient. A total of 12 fractions (850 μL) were collected from the top of the gradient, and absorbance was measured at 260 nm to identify fractions containing monosomes and polysomes. Fraction 1 was devoid of any ribosomes, and fraction 2s−4 were nonpolysomes containing ribosome subunits and monosomes. Polysomes were present in fractions 5−12. (B and C) Distribution of endogenous beta-actin mRNA and exogenous luciferase mRNA in polysomes separated into 12 fractions by sucrose gradient. Blue line: cell lysates from HEK293 cells transfected with DNR 11 repeats; red line: HEK293 cell lysates from HEK293 cells transfected with DNR 13 repeats. (B) Endogenous beta-actin mRNA was found mostly on polysome fractions in both samples indicating active translation. (C) Polysome distribution of luciferase mRNA generated from transfected cells differed between DNR11 and DNR13—with more mRNA on polysomal fractions from DNR11 (47%) than DNR13 (15%).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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