FIGURE

Figure 3

ID

ZDB-FIG-190723-137

Publication

Kawahara et al., 2016 - Site-Specific Integration of Exogenous Genes Using Genome Editing Technologies in Zebrafish

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Figure 3

Site-specific insertion of the hsp promoter-Gal4 donor into the evx2 locus. The donor vector consists of a bait sequence (blue box; target site B for Gbait-gRNA), the hsp promoter (light blue box), a Gal4 driver (pink box) and a polyA signal (pA) (orange box). The target site A for evx2-gRNA (red box) is located in the promoter region of the evx2 gene. When the CRISPR/Cas9 system simultaneously cleaves the target site A and B, the Gal4 driver is unpredictably integrated into the evx2 locus. Not only forward and reverse integrations but also tandem donor vector integrations can occur. We observed eGFP expression in a subset of neurons of the Tg[evx2-hs:Gal4]; Tg[UAS:eGFP] transgenic line because Gal4 driven by the endogenous evx2 enhancer induced eGFP expression as described previously [41]. Gray boxes; exons in the evx2 gene.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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