ZFIN Image: Kawahara et al., 2016, Figure 3

Image

Figure Caption

Figure 3

Site-specific insertion of the hsp promoter-Gal4 donor into the evx2 locus. The donor vector consists of a bait sequence (blue box; target site B for Gbait-gRNA), the hsp promoter (light blue box), a Gal4 driver (pink box) and a polyA signal (pA) (orange box). The target site A for evx2-gRNA (red box) is located in the promoter region of the evx2 gene. When the CRISPR/Cas9 system simultaneously cleaves the target site A and B, the Gal4 driver is unpredictably integrated into the evx2 locus. Not only forward and reverse integrations but also tandem donor vector integrations can occur. We observed eGFP expression in a subset of neurons of the Tg[evx2-hs:Gal4]; Tg[UAS:eGFP] transgenic line because Gal4 driven by the endogenous evx2 enhancer induced eGFP expression as described previously [41]. Gray boxes; exons in the evx2 gene.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.