PUBLICATION
Site-Specific Integration of Exogenous Genes Using Genome Editing Technologies in Zebrafish
- Authors
- Kawahara, A., Hisano, Y., Ota, S., Taimatsu, K.
- ID
- ZDB-PUB-160519-16
- Date
- 2016
- Source
- International Journal of Molecular Sciences 17(5): (Review)
- Registered Authors
- Kawahara, Atsuo, Ota, Satoshi
- Keywords
- CRISPR/Cas9 system, MMEJ, genome editing, knock-in, zebrafish
- MeSH Terms
-
- Animals
- CRISPR-Cas Systems
- Gene Editing/methods*
- Gene Knock-In Techniques/methods*
- Recombination, Genetic
- Zebrafish/genetics*
- PubMed
- 27187373 Full text @ Int. J. Mol. Sci.
Citation
Kawahara, A., Hisano, Y., Ota, S., Taimatsu, K. (2016) Site-Specific Integration of Exogenous Genes Using Genome Editing Technologies in Zebrafish. International Journal of Molecular Sciences. 17(5).
Abstract
The zebrafish (Danio rerio) is an ideal vertebrate model to investigate the developmental molecular mechanism of organogenesis and regeneration. Recent innovation in genome editing technologies, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) system, have allowed researchers to generate diverse genomic modifications in whole animals and in cultured cells. The CRISPR/Cas9 and TALEN techniques frequently induce DNA double-strand breaks (DSBs) at the targeted gene, resulting in frameshift-mediated gene disruption. As a useful application of genome editing technology, several groups have recently reported efficient site-specific integration of exogenous genes into targeted genomic loci. In this review, we provide an overview of TALEN- and CRISPR/Cas9-mediated site-specific integration of exogenous genes in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping